Journal: RSC Advances

Article Title: Dual inhibitory potential of ganoderic acid A on GLUT1/3: computational and in vitro insights into targeting glucose metabolism in human lung cancer †

doi: 10.1039/d4ra04454a

Figure Lengend Snippet: Validation and quality evaluation of the predicted structure of GLUT1 in exofacial conformation. Plot (A) shows the Predicted GLUT1exo model. Ramachandran plot (PROCHECK) of GLUT1exo model (B), and (C) displays ERRAT analysis of GLUT1exo model after energy minimization.

Article Snippet: The polyclonal antibodies for GLUT1 (Cat No. 21829-1-AP), and GLUT3 (Cat No. A5515) were purchased from Proteintech and Selleckchem, respectively.

Techniques: Biomarker Discovery

Journal: RSC Advances

Article Title: Dual inhibitory potential of ganoderic acid A on GLUT1/3: computational and in vitro insights into targeting glucose metabolism in human lung cancer †

doi: 10.1039/d4ra04454a

Figure Lengend Snippet: List of compound identity, binding affinity, and MMGBSA (dG) of ganoderic acid A (GAA), cytochalasin B (CCB), and phloretin (Phlo) with GLUT1/3

Article Snippet: The polyclonal antibodies for GLUT1 (Cat No. 21829-1-AP), and GLUT3 (Cat No. A5515) were purchased from Proteintech and Selleckchem, respectively.

Techniques: Binding Assay

Journal: RSC Advances

Article Title: Dual inhibitory potential of ganoderic acid A on GLUT1/3: computational and in vitro insights into targeting glucose metabolism in human lung cancer †

doi: 10.1039/d4ra04454a

Figure Lengend Snippet: Protein–ligand interactions profile prediction of ligand–GLUT1 complexes. Panels (A and B) show the 3D interactions of GAA (A) and cytochalasin B (B), respectively in a complex with GLUT1endo, while panels (C and D) display the 3D interactions of GAA and phloretin, respectively in a complex with GLUT1exo. The blue line represents the hydrogen bonds. The pink and red represent the nitrogen and oxygen atoms, respectively.

Article Snippet: The polyclonal antibodies for GLUT1 (Cat No. 21829-1-AP), and GLUT3 (Cat No. A5515) were purchased from Proteintech and Selleckchem, respectively.

Techniques:

Journal: RSC Advances

Article Title: Dual inhibitory potential of ganoderic acid A on GLUT1/3: computational and in vitro insights into targeting glucose metabolism in human lung cancer †

doi: 10.1039/d4ra04454a

Figure Lengend Snippet: RMSD and RMSF study plots of ligand–GLUT1 complexes. (A and B) Represent the RMSD plots for the GLUT1endo and GLUT1exo, respectively. While (C and D) represent the RMSF plots, respectively for the GLUT1endo and GLUT1exo. Trajectories for the apo-GLUT1, and complexes with GAA, cytochalasin (CCB), and phloretin (Phlo) are shown in light grey, blue, orange, and cyan colors, respectively.

Article Snippet: The polyclonal antibodies for GLUT1 (Cat No. 21829-1-AP), and GLUT3 (Cat No. A5515) were purchased from Proteintech and Selleckchem, respectively.

Techniques:

Journal: RSC Advances

Article Title: Dual inhibitory potential of ganoderic acid A on GLUT1/3: computational and in vitro insights into targeting glucose metabolism in human lung cancer †

doi: 10.1039/d4ra04454a

Figure Lengend Snippet: RMSD plots of GLUT1/3–ligand complexes. (A and B) Represent ligand RMSD plots for the GLUT3endo and GLUT3exo, respectively. Trajectories for the GLUT1/3-bound GAA in endofacial and exofacial conformation are shown in blue and dark blue, respectively, while cytochalasin (CCB), and phloretin are shown in orange, and cyan colors, respectively.

Article Snippet: The polyclonal antibodies for GLUT1 (Cat No. 21829-1-AP), and GLUT3 (Cat No. A5515) were purchased from Proteintech and Selleckchem, respectively.

Techniques:

Journal: RSC Advances

Article Title: Dual inhibitory potential of ganoderic acid A on GLUT1/3: computational and in vitro insights into targeting glucose metabolism in human lung cancer †

doi: 10.1039/d4ra04454a

Figure Lengend Snippet: The histogram of GLUT1–ligand interactions. (A–D) Plots represent GAA-GLUT1endo, GAA-GLUT1exo, CCB-GLUT1endo, and Phlo-GLUT1exo, respectively. The histogram of protein–ligand interaction displays hydrogen bonding in green color, ionic in red, water bridges in blue, and hydrophobic bonds in violet.

Article Snippet: The polyclonal antibodies for GLUT1 (Cat No. 21829-1-AP), and GLUT3 (Cat No. A5515) were purchased from Proteintech and Selleckchem, respectively.

Techniques:

Journal: RSC Advances

Article Title: Dual inhibitory potential of ganoderic acid A on GLUT1/3: computational and in vitro insights into targeting glucose metabolism in human lung cancer †

doi: 10.1039/d4ra04454a

Figure Lengend Snippet: PCA of top three principal component analysis of 100 ns, along with eigenvalue rank plots. (A–D) Plots represent GAA-GLUT1endo, GAA-GLUT1exo, GAA-GLUT3endo, and GAA-GLUT3exo, respectively. The plot had multiple data points, where each one denoting the conformation of the protein. A color gradient indicates the initial to final stages of the simulation (blue to red for GLUT1 and red to black for GLUT3).

Article Snippet: The polyclonal antibodies for GLUT1 (Cat No. 21829-1-AP), and GLUT3 (Cat No. A5515) were purchased from Proteintech and Selleckchem, respectively.

Techniques:

Journal: RSC Advances

Article Title: Dual inhibitory potential of ganoderic acid A on GLUT1/3: computational and in vitro insights into targeting glucose metabolism in human lung cancer †

doi: 10.1039/d4ra04454a

Figure Lengend Snippet: Cytotoxicity, glucose consumption, and cellular thermal shift assays. Panels (A and B) display the cytotoxicity effect of GAA on A549 and H1299 cells, respectively. Panels (C and D) show the impact of GAA on glucose consumption by A549 and H1299, respectively. Panels (E and F) demonstrate the thermal stability of GLUT1/3 by GAA in A549 and H1299 cells, respectively. Statistical significance is denoted as follows: * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: The polyclonal antibodies for GLUT1 (Cat No. 21829-1-AP), and GLUT3 (Cat No. A5515) were purchased from Proteintech and Selleckchem, respectively.

Techniques:

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Protective Effect of Shengmaiyin in Myocardial Hypertrophy-Induced Rats: A Genomic Analysis by 16S rDNA

doi: 10.1155/2022/3188292

Figure Lengend Snippet: SMY regulates the key signalling pathway in rats with ISO-induced myocardial hypertrophy. (a) ADCY1, PKA, PPAR γ, and Glut4 levels. (b) Relative expression of ADCY1, PKA, PPAR γ, and Glut4. (c) HIF1ɑ, PPARɑ, CPT1A, and Glut1 levels. (d) Relative expression of HIF1 α , PPAR α , CPT1A, and Glut1. n = 3 rats per group. The data are presented as the mean ± SEM; p < 0.05 is considered significant ( # p < 0.05, ## p < 0.01 and ### p < 0.001 compared with the blank group; ∗ p < 0.05, ∗∗ p < 0.01 and ∗∗∗ p < 0.001 compared with the model group).

Article Snippet: Glut1 (Cat no. 21829-1-AP) and PPAR γ (Cat no. 16643-1-AP) primary antibodies were obtained from Proteintech (China).

Techniques: Expressing